Pick the kind of graph with the two large buttons at the top left.
📈 Graph x–y data
Data: type values, or copy cells in Excel, Google Sheets or Logger Pro, click a cell and press Ctrl+V. Choose raw trials to have the mean, SD, SE and 95% CI worked out for you; choose what the error bars show.
Curve fit: linear (slope ± uncertainty), quadratic, exponential, logarithmic, power, saturation (Michaelis–Menten, with or without an offset) or logistic. Each shows its equation and R². There are also max/min slope lines through the error bars and a 95% confidence band.
Statistics: correlation (Pearson, Spearman) and quick comparisons. For a full ANOVA, use Compare groups.
📊 Compare groups
Data: one column per group, its name in the top row, one value per replicate. Paste a whole block, with or without the header row. 📐 turns repeated measurements (e.g. heights on several dates) into % change, total change, rate, final value or mean.
Graph: bar chart (with error bars and the individual values as dots), box-and-whisker plot, dot plot, or means with error bars.
Amounts and trends: when the groups are amounts of one thing (0, 5, 10 mL; 20, 30, 40 °C), tick The groups are amounts of one variable. The groups go on a number line and a trend line is fitted to every replicate. Statistics & report then gives the slope with its 95% CI, R², p and a lack-of-fit test. Don't use it for categories such as species or brands, and decide before you collect the data whether your main test is the trend or the group comparison.
Statistics & report: a descriptive table, checks of normality and equal variances, then the right test (ANOVA, Welch's ANOVA, Kruskal–Wallis; or for two groups a t-test or Mann–Whitney), effect sizes, post-hoc comparisons (Tukey, Games–Howell or Dunn) with letters on the graph, and a written report you can copy and rewrite in your own words.
Labels & style
Title, axis labels with units (use the symbol buttons for ± ° µ ² ⁻¹ ₂ Δ), a figure caption, axis ranges, log scales, colour-blind-safe or greyscale colours, text size and the shape of the graph.
Saving
⬇ PNG for your report (choose 3× or 4× for printing), ⬇ SVG to edit the picture, 📋 Copy image to paste into Word or Google Docs, ⬇ Data + stats for a CSV of the numbers.
Save graph file keeps the graph and its data so you can open it again later (Open…). Your last graph is also remembered in this browser.
Keys: arrow keys and Enter move between table cells.
Italic, bold and underline
Select words in a title, label, caption or group name and press I, B or U (or Ctrl+I / B / U). In the box this shows as *Daphnia magna*, **bold** and __underline__; on the graph it is formatted. Use italics for genus and species names. Choose the font under Labels & style → Font.
Good practice
Label both axes with the quantity and its unit, and say in the caption what the error bars show and how many replicates each point has.
Report the test, the statistic with its degrees of freedom, the exact p-value and an effect size; then say what it means biologically and evaluate the limitations.
The automatic write-up is a starting point. Rewrite it in your own words.
Graph Studio comes from Simulake, a freshwater ecosystem simulator. The statistics were checked against SciPy, a standard scientific library.
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