Pick the kind of graph with the two large buttons at the top left.

📈 Graph x–y data

• Data: type values, or copy cells in Excel, Google Sheets or Logger Pro, click a cell and press Ctrl+V. Choose raw trials to have the mean, SD, SE and 95% CI worked out for you; choose what the error bars show.
• Curve fit: linear (slope ± uncertainty), quadratic, exponential, logarithmic, power, saturation (Michaelis–Menten, with or without an offset) or logistic. Each shows its equation and R². There are also max/min slope lines through the error bars and a 95% confidence band.
• Statistics: correlation (Pearson, Spearman) and quick comparisons. For a full ANOVA, use Compare groups.

📊 Compare groups

• Data: one column per group, its name in the top row, one value per replicate. Paste a whole block, with or without the header row. 📐 turns repeated measurements (e.g. heights on several dates) into % change, total change, rate, final value or mean.
• Graph: bar chart (with error bars and the individual values as dots), box-and-whisker plot, dot plot, or means with error bars.
• Amounts and trends: when the groups are amounts of one thing (0, 5, 10 mL; 20, 30, 40 °C), tick The groups are amounts of one variable. The groups go on a number line and a trend line is fitted to every replicate. Statistics & report then gives the slope with its 95% CI, R², p and a lack-of-fit test. Don’t use it for categories such as species or brands, and decide before you collect the data whether your main test is the trend or the group comparison.
• Statistics & report: a descriptive table, checks of normality and equal variances, then the right test (ANOVA, Welch’s ANOVA, Kruskal–Wallis; or for two groups a t-test or Mann–Whitney), effect sizes, post-hoc comparisons (Tukey, Games–Howell or Dunn) with letters on the graph, and a written report you can copy and rewrite in your own words.

Labels & style

• Title, axis labels with units (use the symbol buttons for ± ° µ ² ⁻¹ ₂ Δ), a figure caption, axis ranges, log scales, colour-blind-safe or greyscale colours, text size and the shape of the graph.

Saving

• ⬇ PNG for your report (choose 3× or 4× for printing), ⬇ SVG to edit the picture, 📋 Copy image to paste into Word or Google Docs, ⬇ Data + stats for a CSV of the numbers.
• Save graph file keeps the graph and its data so you can open it again later (Open…). Your last graph is also remembered in this browser.
• Keys: arrow keys and Enter move between table cells.

Italic, bold and underline

• Select words in a title, label, caption or group name and press I, B or U (or Ctrl+I / B / U). In the box this shows as *Daphnia magna*, **bold** and __underline__; on the graph it is formatted. Use italics for genus and species names. Choose the font under Labels & style → Font.

Good practice

• Label both axes with the quantity and its unit, and say in the caption what the error bars show and how many replicates each point has.
• Report the test, the statistic with its degrees of freedom, the exact p-value and an effect size; then say what it means biologically and evaluate the limitations.
• The automatic write-up is a starting point. Rewrite it in your own words.

Graph Studio comes from Simulake, a freshwater ecosystem simulator. The statistics were checked against SciPy, a standard scientific library.

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